ESPN 50th Annual Meeting

ESPN 2017


 
Micrоbiologiсal structure of urinary tract infection in children in Orenburg region
ALBINA VYALKOVA IGOR ZORIN 1 VIKTOR GRITSENKO 1 SVETLANA TCHESNOKOVA 1 SVETLANA DREBEZOVA 1 ELENA GUNKOVA 1 ALYONA AKHMETGALEEVA 2

1- ORENBURG MEDICAL UNIVERSITY
2- PAEDIATRC CLINIC №6
 
Introduction:

 The aim of the study was to establish micribiological structure of urinary tract infection (UTI) in children in Orenburg region. 

Material and methods:

 We examined 6392 cases of children in period 2006-2016 years who treated with UTI in paediatric clinic №6 in Orenburg region. There were 3451 (53,9%) girls and 2941 (46,1%) boys from 1 till 15 years. All children underwent special microbiological examination of urine including determination of degree of bacteriuria sectoral sowing on blood agar medium and Endo (Feldman J.M. et al., 1984) and quantitative parameters (Grachev N. et al ., 1986). Species identification allocated urine’s bacteria carried out by conventional methods (Birger M.I., 1982). We determined persistence markers of isolated strains of microorganisms: antilysozyme activity (ALA), antiinterferon activity (AIA) and seroresistance (SR) (Bukharin O.V. et al, 1996).

Results:

 We established that E. coli prevailed in microbiological structure of UTI (78,9%, n=5049). Proportion of other bacteria which were isolated from urine was significantly lower (21,1%, n=1343): Klebsiella (16,8%, n=1080), Proteus (4,3%, n=263). We studied also  resistance of bacteria isolated from urine to antibiotics. Escherichia coli were resistance to ampicillin (51,5%), amoxicillin (51,5%), 1-st generations of cephalosporins (46%) and penicillins with clavulanic acid (35,5%).  Klebsiella were 100% resistant to ampicillin and amoxicillin, penicillins with clavulanic acid (30%), 1-st generations of cephalosporins  (56%), furans (40%). Proteus exhibit low sensitivity to furans (resistance 45%), ampicillin and amoxycillin (74%), 1-st generations of cephalosporins (100%), 2-nd generations of cephalosporins (38%), 100% to penicillins with clavulanic acid. We determined that microorganism strains isolated from urine of patients with UTI were characterized by high levels of SR, ALA, AIA.

Conclusions:

 These data were used in creation of regional register of antimicrobial resistance paediatric patients with UTI. High levels of SR, ALA, AIA show high persistent and pathological potential of urine’s bacteria in pathogenesis of UTI.