ESPN 50th Annual Meeting

ESPN 2017


 
Matrix Metalloproteinases in infants with Ureteropelvic Junction Obstruction
ANTIGONE PAVLAKI 1 NIKOLETA PRINTZA 1 EVANGELIA FARMAKI 1 ANNA TAPARKOU 1 STYLIANI STABOULI 1 JOHN DOTIS 1 CHRYSA GKOGKA 1 FOTIOS PAPACHRISTOU 1

1- ARISTOTLE UNIVERSITY OF THESSALONIKI, 1ST PEDIATRIC DEPARTMENT
 
Introduction:

The management of patients with ureteropelvic junction obstruction (UPJO) remains a controversial issue. The objective is the preservation of renal function through early selection of patients who will require surgical intervention as opposed to the others who will be monitored systematically by various imaging methods. Aim of the present study was to measure Matrix Metalloproteinases (MMPs) levels in UPJO patients who were planned to undergo surgery using standard criteria and thus clarify if MMPs levels could serve as potential biomarkers of obstruction in hydronephrosis. 

Material and methods:

Serum samples of infants with UPJO diagnosis who were planned to undergo surgery were compared to 17 serum samples of healthy age matched controls. MMP2 and MMP9 were quantified using immunoenzymatic (ELISA) assay. 

Results:

17 infants with UPJO diagnosis, 14 males and three females, median age 1.5 months (min: one month, max: 20 months), mean anteroposterior diameter in the hydronephrotic kidney 23.4mm (min: 19mm, max: 50mm) and T ½ > 20 min in the MAG3 renogramm were recruited. MMP9 levels were significantly decreased in serum samples of UPJO patients (418.53 ng/ml +/- 256.78 ng/ml) vs control levels (572 ng/ml +/- 226.96 ng/ml) (p= 0.037). MMP2 values were higher in UPJO patients (502.2 ng/ml +/- 64.2 ng/ml) vs controls (471.42 ng/ml +/- 123.35 ng/ml). However, the difference was not statistically significant (p= 0.206). 

Conclusions:

This study presents decreased concentrations of MMP9 in the serum of patients with obstructive hydronephrosis but the results should be tested in larger population samples and even be evaluated simultaneously with urine samples in order to delineate MMPs ability to serve as obstruction biomarkers.